b)-Temperature:-increase in temperature increases rate of enzymatic activity up to an optimum/ low
temperature inactivates the enzymes/high temperatures above #40^0# C denatures enzymes/ enzymatic activity and at optimum temperature enzymes work at its best hence maximum/highest rate of reaction.
-PH:-Enzymes work best at optimum
PH/extreme PH denatures enzymes. Some enzymes act best in acidic or basic medium. Optimum PH should be maintained.
-Enzyme concentration:-Increase in concentration increases enzymatic activity as there is more active sites to combine with substrate.
-Co-enzymes:-activates enzymes increasing rate of activity.
-Substrate concentration:-increase in concentration increases enzymatic activity up to certain level where enzymes become a limiting factor.
-Inhibitors:-compete with substrate for active sites or combines permanently with active sites of enzymes. They slow down or stop the rate of reaction